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Image Search Results
Journal: The Journal of Cell Biology
Article Title: Quality control of mitochondrial protein synthesis is required for membrane integrity and cell fitness
doi: 10.1083/jcb.201504062
Figure Lengend Snippet: Actinonin impairs the turnover of de novo mitochondrial protein synthesis. (A, left) Standard protocol for metabolic labeling of HEK293 cells after ATP5B ( A-5B ) siRNA knockdown or scrambled control (SC) with a chase. Arrows indicate the position of MT-ATP6 and MT-ATP8. (right) SDS-PAGE immunoblot of whole cell lysates with representative antibodies. (B) Modified protocol for extended metabolic labeling of HEK293 cells after ATP5B siRNA knockdown for a 3-h pulse and chase in the indicated conditions (Act, actinonin; E, ethanol). (C) Immunoblot of whole cell lysates of ATP5B siRNA knockdown treated with actinonin or ethanol for 6 h. (D) HEK293 cells metabolically labeled for 45 min with [ 35 S]methionine/cysteine with and without actinonin and then precipitated with CTAB. Each sample was split equally in two with one half treated with RNase A before addition of CTAB. A representative image of three independent experiments is shown.
Article Snippet: Rabbit anti-Mrpl13 was a gift from N. Göran Larsson ( ); from the
Techniques: Labeling, SDS Page, Western Blot, Modification, Metabolic Labelling